Effects of Derivatization on the Metabolite Profiling of the Cadmium-Tolerant Mangrove Fungus Trichoderma atroviride Using GC-MS Analysis

The mangrove fungus Trichoderma atroviride was found to be tolerant the heavy metal cadmium and it is of high interest profile its metabolites gain insight into response toxicity. This study aimed investigate effect derivatization agents on number types present in cadmium-tolerant T. atroviride, detected using GC-MS analysis. intracellular extracellular treated with for ten days were derivatized silylation alkylation reactions. results showed that a higher identified when three different used: BSTFA, TBDMSTFA, MCF. More by silylation, making suitable non-targeted profiling study. Silylation efficient analysis sugars their derivatives while targeted involving amino acids organic acids. Statistical data set performed Metaboanalyst 3.0 followed visualization Partial Least Square-Discriminant Analysis. plots clear separations between control cadmium-treated samples. A more comprehensive metabolite obtained this study, distinct samples, will provide good baseline information future investigations including pathways biomarkers responsible fungal tolerance